Thursday, 3 January 2013

2.11 describe experiments to investigate how enzyme activity can be affected by changes in temperature.


  • Put starch into a test tube; either heat or cool it.
  • Add amylase
  • With this mixture on white tiles, add iodine
  • Time how long it takes for the iodine to stop being blue black
  • Repeat at different temperatures and compare
When the iodine stops being blue/black there is no starch present, so it must have been digested by the enzymes.

24 comments:

  1. will the product be a simple sugar?

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    Replies
    1. Yes, because amylase breaks down starch into the sugar maltose, which is a simple sugar.

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    2. Yes that's right, thanks for helping :)

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  2. What colour will the iodine turn when it stops being blue/black??

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  3. Doesnt it have to change to Blue Black, because it is Iodine??? Red to Blue Black, it said in like 2.7 of your blog???

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  4. Doesnt it have to change to Blue Black, because it is Iodine??? Red to Blue Black, it said in like 2.7 of your blog???

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  5. It will start off as blue/black as there is starch in the solution however you will know when there is no more starch as the iodine in the solution will turn a red/brown colour

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  6. Must you add the amylase after the glucose is heated?

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  7. HANNAH!!! YOU ARE AMAZING!!!THIS BLOG IS THE REASON IM NOT SO STRESSED OUT! PLEASE KEEP GOING, KNOWING THAT YOU HAVE HELPED ME SO MUCH.....THANK YOU THANK YOU THANK YOU!!!!

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  8. The Biology Syllabus has changed.... New Syllabus Blog:

    http://igcse-biology-2017.blogspot.hk

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  9. more at

    http://igcse-biology-notes.blogspot.hk/2017/06/211-describe-experiments-to-investigate.html

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  10. Is it fine if I wrote
    Enzyme catalase ,which breaks hydrogen peroxide into water and hydrogen
    Catalase in found in potatoes , therefore putting potato chips into preoxide will produce O2 .the rate of reaction is therefore proportional to the volume of 02 given off. The changing in temperature will alter the volume

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